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MetaMorph Inc integrative morphometry analysis software
Integrative Morphometry Analysis Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrated+morphometry+software/morphometry+software/10__1523_slash_jneurosci__2264___22__2023-87-39-42
Average 90 stars, based on 1 article reviews
integrative morphometry analysis software - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:

Article Title: The thrombospondin-1 receptor CD36 is an important mediator of ovarian angiogenesis and folliculogenesis
Article Snippet: .. For CD31 staining, blood vessel density was calculated as the percentage of ovarian tissue comprised of CD31-postive endothelium using integrated morphometry software (Metamorph, CA). ..

Article Title: Thrombospondin-1 Inhibits VEGF Levels in the Ovary Directly by Binding and Internalization Via the Low Density Lipoprotein Receptor-Related Protein-1 (LRP-1)
Article Snippet: Cells were imaged with an inverted Olympus microscope using phase contrast to demonstrate cell morphology. .. Fluorescence staining was captured, and overlay of immunofluorescence and phase contrast images was performed using integrated morphometry software (Metamorph). ..

Software:

Article Title: The thrombospondin-1 receptor CD36 is an important mediator of ovarian angiogenesis and folliculogenesis
Article Snippet: .. For CD31 staining, blood vessel density was calculated as the percentage of ovarian tissue comprised of CD31-postive endothelium using integrated morphometry software (Metamorph, CA). ..

Article Title: Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss.
Article Snippet: Antigen visualization was performed using DAB in solution (SigmaFast 3,3’-Diaminobenzidine tablets), and tissues were counterstained with Carazzi’s Hematoxylin (Fisher Scientific, H34525), mounted on coverslips (Fisher Scientific, 12-548-5E) using Permount Mounting Medium (Fisher Chemical, SP15-100, Ottawa, Canada) and images captured using brightfield microscopy (Nikon Eclipse E600, Qimaging mono 12 bit camera). .. The percentage of immunopositive cells was semi-quantified using integrated morphometry software (Metamorph, CA) with a minimum of five fields of view per tissue. .. Molecular analyses RNA extraction of neonatal (P4) ovaries Total RNA was extracted from formalin fixed ovaries using an RNeasy FFPE Kit (Qiagen, 735504) following the manufacturer’s instructions.

Article Title: Delta-9-tetrahydrocannabinol increases vascular endothelial growth factor (VEGF) secretion through a cyclooxygenase-dependent mechanism in rat granulosa cells.
Article Snippet: While the effects of delta-9-tetrahydrocannabinol (THC), the psychoactive component of cannabis, have been studied extensively in the central nervous system, there is limited knowledge about its effects on the female reproductive system.. The aim of this study was to assess the effect of THC on the expression and secretion of the angiogenic factor vascular endothelial growth factor (VEGF) in the ovary, and to determine if these effects were mediated by prostaglandins.. Spontaneously immortalized rat granulosa cells (SIGCs) were exposed to THC for 24 h. Gene expression, proliferation and TNFα-induced apoptosis were evaluated in the cells and concentrations of VEGF and prostaglandin E2 (PGE2), a known regulator of VEGF production, were determined in the media.

Article Title: Prenatal Exposure to Delta-9-tetrahydrocannabinol (THC) Alters the Expression of miR-122-5p and Its Target Igf1r in the Adult Rat Ovary
Article Snippet: .. The percentage of immunopositive follicular cells was quantified by the same individual, who was blinded to the treatment group until all the data had been collected, with the use of an integrated morphometry software (MetaMorph Inc., Nashville, TN, USA). ..

Article Title: Fetal and neonatal exposure to nicotine disrupts postnatal lung development in rats: Role of VEGF and its receptors
Article Snippet: .. Blood vessel density was quantified using integrated morphometry software (MetaMorph, CA) in which the number of blood vessels per field of view were counted (n=6 animals per group, 5 fields of view per animal). ..

Article Title: The impact of the ovarian microenvironment on the anti-tumor effect of SPARC on ovarian cancer.
Article Snippet: .. Microvessel area was measured relative to the total tissue area using integrated morphometry software (Metamorph). ..

Article Title: Thrombospondin-1 Inhibits VEGF Levels in the Ovary Directly by Binding and Internalization Via the Low Density Lipoprotein Receptor-Related Protein-1 (LRP-1)
Article Snippet: Cells were imaged with an inverted Olympus microscope using phase contrast to demonstrate cell morphology. .. Fluorescence staining was captured, and overlay of immunofluorescence and phase contrast images was performed using integrated morphometry software (Metamorph). ..

Article Title: The thrombospondin-1 receptor CD36 is an important mediator of ovarian angiogenesis and folliculogenesis
Article Snippet: After rinsing, cells were stained with 2ug/ml DAPI (Sigma) to counterstain nuclei blue and mounted on glass slides (SuperFrost Plus, Fisher) with Prolong Gold antifade solution (Invitrogen). .. Epifluorescence microscopy was used for image acquisition and integrated morphometry software (Metamorph, Burlingname, CA) was used to quantify the percent immunopositive cells in follicles without (pre-antral) or with (antral) an antrum, and in corpora lutea. .. Five micrometer-thick paraffin embedded ovarian tissue sections from wild-type and CD36 -\- mice were incubated overnight at 4°C in a humidified chamber with rabbit polyclonal anti-VEGF antibody (1:600 dilution; Santa Cruz Biotechnology, CA, sc152), rabbit polyclonal anti-VEGFR-2 antibody (1:200 dilution; Cell Signaling, 2479); goat polyclonal anti-TSP-1 antibody (1:600 dilution; Santa Cruz, sc59887); mouse monoclonal anti-CD31 (1:500 dilution; Abcam; ab28634); or mouse monoclonal anti-Ki67 antibody (1:500 dilution; Sigma, Oakville, ON, SAB4501880).

Fluorescence:

Article Title: Thrombospondin-1 Inhibits VEGF Levels in the Ovary Directly by Binding and Internalization Via the Low Density Lipoprotein Receptor-Related Protein-1 (LRP-1)
Article Snippet: Cells were imaged with an inverted Olympus microscope using phase contrast to demonstrate cell morphology. .. Fluorescence staining was captured, and overlay of immunofluorescence and phase contrast images was performed using integrated morphometry software (Metamorph). ..

Immunofluorescence:

Article Title: Thrombospondin-1 Inhibits VEGF Levels in the Ovary Directly by Binding and Internalization Via the Low Density Lipoprotein Receptor-Related Protein-1 (LRP-1)
Article Snippet: Cells were imaged with an inverted Olympus microscope using phase contrast to demonstrate cell morphology. .. Fluorescence staining was captured, and overlay of immunofluorescence and phase contrast images was performed using integrated morphometry software (Metamorph). ..

Epifluorescence Microscopy:

Article Title: The thrombospondin-1 receptor CD36 is an important mediator of ovarian angiogenesis and folliculogenesis
Article Snippet: After rinsing, cells were stained with 2ug/ml DAPI (Sigma) to counterstain nuclei blue and mounted on glass slides (SuperFrost Plus, Fisher) with Prolong Gold antifade solution (Invitrogen). .. Epifluorescence microscopy was used for image acquisition and integrated morphometry software (Metamorph, Burlingname, CA) was used to quantify the percent immunopositive cells in follicles without (pre-antral) or with (antral) an antrum, and in corpora lutea. .. Five micrometer-thick paraffin embedded ovarian tissue sections from wild-type and CD36 -\- mice were incubated overnight at 4°C in a humidified chamber with rabbit polyclonal anti-VEGF antibody (1:600 dilution; Santa Cruz Biotechnology, CA, sc152), rabbit polyclonal anti-VEGFR-2 antibody (1:200 dilution; Cell Signaling, 2479); goat polyclonal anti-TSP-1 antibody (1:600 dilution; Santa Cruz, sc59887); mouse monoclonal anti-CD31 (1:500 dilution; Abcam; ab28634); or mouse monoclonal anti-Ki67 antibody (1:500 dilution; Sigma, Oakville, ON, SAB4501880).



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